Installation of the first benchtop automated HMW DNA extraction from Fire Monkey at the Quadram Institute for Pathogen Sequencing and AMR Monitoring
LONDON, July 8, 2022 /PRNewswire/ — British genomics company, RevoluGen Ltd. (RevoluGen or the Company), is pleased to announce that she has been selected as the winner of the BioNewsRound Awards 2022for the development and automation of its Fire Monkey High Molecular Weight (HMW) DNA extraction system and successful transfer to its collaborator, Quadram Institute Biosciences (Quadram).
The award, organized by leading trade association One Nucleus, aims to celebrate the life sciences successes of the past year. Concretely, this translational prize, organized as part of the On Helix conference in Cambridgerewards companies that have successfully advanced their agenda along the innovation pipeline, whether it is a new therapeutic, diagnostic or enabling technology.
RevoluGen has invested over 12 months in automating its technology enabling the extraction of HMW Fire Monkey DNA in a standard 96-well filter plate format, the world’s first automated extraction of library-ready HMW DNA. The extraction protocol has been validated for extracting HMW DNA from bacterial and mammalian samples, and the collaboration with Quadram is the company’s first customer deployment.
End December, RevoluGen announced that it was transferring a Fire Monkey automated system robot to Quadram, a leading UK research institute, to enable automation of high molecular weight (HMW) DNA extraction in two high molecular weight bacterial sequencing projects volume in monitoring pathogen persistence and antimicrobial resistance (AMR).
Fire Monkey HMW Automated DNA Extraction is used in Quadram’s long-read DNA sequencing programs based on high-quality hybrid constructs initially in two key projects: processing Salmonella samples taken by a UK Health and Safety Agency (UKHSA) laboratory; and treatment Salmonella and Campylobacter samples of the Norfolk area that is interested in, among other things, antimicrobial resistance (AMR). In 2022, Quadram and UKHSA scientists presented data at several scientific meetings and published several peer-reviewed articlesusing Fire Monkey to extract the DNA.
Doctor Tony JonesCEO of One Nucleus and organizer of the BioNewsRound Award said“When selecting finalists, we look for the impact of the news on the development of the organization, the importance for the life sciences industry as a whole and the potential benefit for patients. RevoluGen has obtained excellent results for all of these criteria.Its technology automates the first step in the DNA sequencing process – extracting DNA from the cell, and its automated protocol will further accelerate high-volume genomics applications. worthy of the name, and I am delighted that he was chosen by his peers as the 2022 winner.”
RevoluGen was selected as a finalist from over 40 entrants and announced as the winner yesterday at the end of the On Helix conference. The other five 2022 finalists were:
RevoluGen CEO Pieter Haitsma Mulier said“We are thrilled to win this award. Automation represents a game-changing differentiator and will allow us to quickly scale sequencing at a lower unit cost. Now that we have validated the automated protocol and have our first installed base, we believe Fire Monkey is fast becoming the DNA extraction technology of choice for high-volume applications.Our technology combines short-read and long-read sequencing, enabling both from the same sample. It offers the flexibility to revisit the same original sample for read sequencing after a first run with cheaper short-read sequencing. The DNA sequencing market is currently expected to shift from $4 billion to some $50 billion over the next 10 years. and RevoluGen is well positioned to make a significant contribution in this space.”
RevoluGen’s patented technology is derived from a spin-column-based protocol to extract HMW DNA using high g-force that does not break up long, fragile DNA molecules. Fire Monkey produces DNA fragments that are neither too short nor too long for long-read sequencing technologies. This size-adjusted DNA fragment extract improves the overall sequencing results of technologies by not wasting sequencing resources by reading less useful small fragments or reducing sequencing throughput by clogging sequencing pores with too many fragments. long.
Doctor Georgios Patsosinventor of Fire Monkey technology and CSO at RevoluGen said: “HMW DNA extraction is the essential first step in the DNA sequencing workflow. The rapid growth of sequencing around the world has driven the need for automation to manage the volume of samples needed for applications such as population genomics, screening for epidemiological mutations, and monitoring microbial resistance genes to antibiotics. Gemma and the Quadram teams are undertaking cutting-edge projects in DNA sequencing and pathogen genomics, and we are excited to deploy the first automated Fire Monkey HMW DNA kits with them. »
Doctor Gemma Langridgea group leader at Quadram, who was an early adopter of RevoluGen’s Fire Monkey technology, said: “Revolugen’s automated workflow is a real improvement. Previously, we extracted DNA from each sample individually using tubes. The robot uses positive air pressure to push the sample through a filtration column in a multi-well plate format.
Steven RudderLangridge Group doctoral student, added, “Before, the fragments were around 300 base pairs. Now they are 10,000 bases, even up to more than 100,000. It’s like having a puzzle. Before, we had a lot of small pieces to put together, now we have large parts that are easier to assemble. We’re excited to be RevoluGen’s first install of its benchtop automated process. There’s always a learning curve with a new install, so I’m troubleshooting using the robot I went from 24 samples in a day being pretty strenuous to now doing 96 in the morning and feeling like I was flying through samples.”
Steven’s PhD project focuses on the next step of scaling and accelerating the process. Steven brings his expertise acquired within the sequencing team of the Quadram Institute. The project is an iCASE fellowship with Revolugen focusing on using a robot to perform the Fire Monkey DNA extraction process so the team can process samples faster.
Read the Quadram institutes blog HERE.
Legend: Steven Rudderdoctoral student in At Gemma Langridge’s research group at the Quadram Institute, Norwich Gives a Boost to RevoluGen’s Fire Monkey HMW-DNA Automated Extraction
Copyright: Quadram Institute
About RevoluGen – www.revolugen.co.uk.
RevoluGen is a privately held scientific research and development company that commercializes molecular tools with a particular focus on the rapid extraction of long, pure DNA fragments from cells.
Revolugen’s Nucleic Acid Isolation and Purification (NAIP) products have wide application in all short and long read DNA sequencing technologies, including their use in particular for population genomics, screening for epidemiological mutations and monitoring of microbial resistance genes to antibiotics.
Fire Monkey dramatically improves sequencing results by extracting long fragment DNA with an in-protocol concurrent size selection feature that minimizes small fragment DNA contamination.
The company’s head office, R&D, manufacturing, direct sales and customer support are based in the UK. RevoluGen serves customers worldwide and has agreements with leading molecular biology tool companies including Merck KGaA (sales), Welgene (distribution), Cytiva (manufacturing), Tecan (automation) and A4P ( logistics).
About the Quadram Biosciences Institute – https://quadram.ac.uk/
The Quadram Institute is an interdisciplinary research center at the forefront of a new era of food and health research. It brings together researchers and clinicians under one roof and houses one of the Europe larger endoscopy units and a clinical research center.
Based at Norwich Research Park, the Quadram Institute is a partnership between the Quadram Institute Bioscience, the Norfolk and Norwich University Hospitals NHS Foundation Trust, University of East Anglia and the Biotechnology and Biological Sciences Research Council (BBSRC).
Its mission is to deliver healthier lives through innovation in gut health, microbiology and nutrition and its vision is to understand how food and microbes interact to promote health and prevent disease.
Quadram Institute Bioscience’s interconnected research themes provide a research pipeline in plants, microbes, food and health: microbes in the food chain; the gut and the microbiome; and food innovation.
About Nucleus – www.onenucleus.com
One Nucleus is a non-profit Life Sciences & Healthcare organization based in Cambridge. We support life sciences institutions, businesses and individuals by providing local, UK-wide and international connectivity.
By providing local, UK-wide and international connectivity, One Nucleus seeks to enable our members to maximize their performance. This support helps them achieve, or even exceed, the objectives they have set themselves. Biomedical and medical R&D has always had an impact on social and economic progress. In an increasingly outsourced, collaborative, and multidisciplinary industry, bringing together the best people is key to translating great innovation into great products that dramatically improve patient outcomes and drive economic development. Attracting and enabling the best people to engage is core to the One Nucleus team philosophy and what we continually strive to deliver.
For more information or to arrange media interviews, please contact:
Sue Charlesdirector and head of corporate communications, RevoluGen
Tel: +44 (0)7968 726585, E: [email protected]
For more information on awards, please contact:
E-mail: [email protected]
Such. : +44 (0)1223 896466
SOURCE RevoluGen Ltd.